Introduction
R&D Systems Rat Cytokine Array (Catalog # ARY008) is a rapid, sensitive, and economical tool to simultaneously detect the relative expression of 29 cytokine and chemokines in a single sample. No specialized equipment is necessary.
- Product Insert (PDF, 186K)
- Protocol for near-infrared (NIR) fluorescence detection
- Troubleshooting Guide
- Assays for analytes represented in the Rat Cytokine Array Kit
| Simultaneously detect the levels of these cytokines in a single sample. | ||
| CXCL1/CINC-1 | IL-1ra | L-Selectin |
| CXCL3/CINC-2 alpha/beta | IL-2 | CXCL9/MIG |
| CINC-3 | IL-3 | CCL3/MIP-1 alpha |
| CNTF | IL-4 | CCL20/MIP-3 alpha |
| Fractalkine/CXC3CL1 | IL-6 | CCL5/RANTES |
| GM-CSF | IL-10 | CXCL7/Thymus Chemokine |
| ICAM-1 | IL-13 | TIMP-1 |
| IFN-gamma | IL-17 | TNF-alpha |
| IL-1 alpha | CXCL10/IP-10 | VEGF |
| IL-1 beta | LIX | |
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, serum, or plasma samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.
Kit Contents
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.